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ansA [2019-02-24 17:46:03]
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ansA [2019-02-24 17:46:03]

L-asparaginase
locus
BSU23580
pI
4.45
mw
36.30 kDa
protein length
329 aa Sequence Blast
gene length
990 bp Sequence Blast
function
asparagine degradation
product
L-asparaginase
essential
no
ec
3.5.1.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]

    • Gene

    Coordinates
    2,455,819 → 2,456,808

    The protein

    Catalyzed reaction/ biological activity

  • L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)

    • Protein family

  • asparaginase 1 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|AnsZ]

    • Structure

  • [PDB|5OT0] (from Thermococcus kodakarensis, 43% identity) [pubmed|29095161]

    • Expression and Regulation

    Operons

    genes
    [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
    description
    [Pubmed|1711029]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1711029], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|AnsR]: repression, [Pubmed|11914346], in [regulon|146947C974D62BCB282BA3F5B924B35695D75886|AnsR regulon]

    • regulation

  • expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
    • view in new tab

    genes
    [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
    description
    [Pubmed|22383849]

    regulation

  • ''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
    • view in new tab

    Biological materials

    Mutant

  • GP1153 (del ''ansAB''::''ermC'') available in the [SW|Stülke] lab
  • BKE23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
  • BKK23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC

    • lacZ fusion

  • pGP872 (in [protein|search|pAC5]), available in [SW|Stülke] lab

    • References

  • 8478318,1711029,11914346,22383849,29095161